Vitamin D Test Solutions At Home Vitamin D Self Test Vitamin D Blood Test
Home blood Vitamin D rapid test kit[INTENDED USE]
The Vitamin D Semi-Quantitative Rapid Test Device (Whole Blood/Serum/Plasma) is used for semi-quantitative determination and monitoring of 25-OH Vitamin D concentrations in whole blood/serum/plasma specimens.
[PRINCIPLE]
The test sample which is diluted with buffer solution add to the sample well of the test device. The sample now moves through the test strip from bottom to top. If the test sample contains 25-OH Vitamin D, it attaches to the first anti-25-OH Vitamin D antibody which is conjugated with a red gold colloidal for color marking. The red 25-OH Vitamin D antibody-gold complex, together with the sample liquid, diffuses through the membrane that is pre dispensed with lines of different amounts of the second anti-25-OH Vitamin D antibody. The 25-OH Vitamin D-antibody-gold complex is immobilized by the antibodies coated on the membrane leading to the formation of red lines. The number of lines depends on the 25-OH Vitamin D concentration in the sample. The control line serves as a procedural control and indicates that sufficient volume of specimen has been added and membrane wicking has occurred.
[STORAGE AND STABILITY]
Store as packaged in the sealed pouch at temperature (4-30℃ or 40-86℉). The kit is stable within the expiration date printed on the labeling.
Once open the pouch, the test should be used within one hour. Prolonged exposure to hot and humid environment will cause product deterioration.
The LOT and the expiration date were printed on the labeling.
[SPECIMEN]
Whole Blood/Serum/Plasma
[TEST PROCEDURE]
- Allow the test device and specimens to equilibrate to temperature (15-30℃or 59-86℉) prior to testing.
- [For Strip]
1. Remove the test strip from the sealed pouch and use it as soon as possible.
2. Place the test strip on a clean and level surface.
3. For serum or plasma specimen: Hold the dropper vertically and transfer 3 drops of serum or plasma (approximately 100μl) to the specimen pad of the test strip, then start the timer. See illustration below.
4. for whole blood specimens: Hold the dropper vertically and transfer 1 drop of whole blood (approximately 35μl) to the specimen pad of the test strip, then add 2 drops of buffer (approximately 70μl) and start the timer. See illustration below.
5. Wait for the colored line(s) to appear. Read results at 15 minutes. Do not interpret the result after 20 minutes.
1.Remove the test cassette from the sealed pouch and use it as soon as Possible.
2.Place the test cassette on a clean and level surface.
3.For serum or plasma specimen: Hold the dropper vertically and transfer 3 drops of serum or plasma (approximately 100μl) to the specimen well (S) of the test cassette, then start the timer. See illustration below.
4.For whole blood specimens: Hold the dropper vertically and transfer 1 drop of whole blood(approximately 35μl) to the specimen well(S) of the test cassette, then add 2 drops of buffer (approximately 70μl) and start the timer. See illustration below.
5.Wait for the colored line(s) to appear. Read results at 15 minutes. Do not interpret the result after 20 minutes.
[INTERPRETATION OF RESULTS]
Positive:*Two lines appear. One colored line should be in the control region (C), and another apparent colored line adjacent should be in the test region (T). This positive result indicates the presence of antigens to Dengue.
Negative: One colored line appears in the control region (C). No line appears in the test region (T). This negative result indicates the absence of antigens to Dengue.
Invalid: Control line fails to appear. Insufficient specimen volume or incorrect procedural techniques are the most likely reasons for control line failure. Review the procedure and repeat the test using a new test cassette/strip. If the problem persists, discontinue using the lot immediately and contact your local distributor.
